growth promotion test procedure Things To Know Before You Buy
A seed-stock method must be utilized for lengthy-expression storage of cultures. Cultures gained in the ATCC should be resuscitated In accordance with directions. If grown in broth, the cells are pelleted by centrifugation. Resuspend in 1/20th the quantity of fresh servicing broth, and include an equal volume of 20% (v/v in drinking water) sterile